Creating Single-Copy Genetic Circuits

Creating Single-Copy Genetic Circuits.

Synthetic biology is increasingly used to develop sophisticated living devices for basic and applied research. Many of these genetic devices are engineered using multi-copy plasmids, but as the field progresses from proof-of-principle demonstrations to practical applications, it is important to develop single-copy synthetic modules that minimize consumption of cellular resources and can be stab...

Optimization of Quantum Cellular Automata Circuits by Genetic Algorithm

Quantum cellular automata (QCA) enables performing arithmetic and logic operations at the molecular scale. This nanotechnology promises high device density, low power consumption and high computational power. Unlike the CMOS technology where the ON and OFF states of the transistors represent binary information, in QCA, data is represented by the charge configuration. The primary and basic devic...

single - copy plasmids for auxotrophy

Auxotrophic markers are useful tools in cloning and genome editing, enable a large spectrum of genetic techniques, as well as facilitate the study of metabolite exchange interactions in microbial communities. If unused background auxotrophies are left uncomplemented however, yeast cells need to be grown in nutrient supplemented or rich growth media compositions, which precludes the analysis of ...

tochastically driven genetic circuits

Transcriptional regulation in small genetic circuits exhibits large stochastic fluctuations. Recent experiments have shown that a significant fraction of these fluctuations is caused by extrinsic factors. In this paper we review several theoretical and computational approaches to modeling of small genetic circuits driven by extrinsic stochastic processes. We propose a simplified approach to thi...

Single-rail handshake circuits